This video shows how to calibrate from manual reference locations. For the calibration sequence, the participant was always looking at the finger tip. There are no markers in this video. This is a Pupil Mobile recording, so it does not contain pupil detections. Therefore, we first detect these. Check out the video on offline pupil detection for more information. To produce gaze, open the Gaze Producers plugin … … and select “Gaze From Offline Calibration” as the gaze producer. To enter manual reference locations, open the “Reference Locations” submenu. Next, turn on the Manual Edit Mode. Click on the video, to set the reference location for the frame. Click elsewhere, to reposition it. Click on the reference location, to delete it. Now we seek to the beginning of the calibration sequence. Let’s hide all timelines. They can occlude reference locations. Now we click at the finger in different frames to set reference locations. Go to the next frame with your arrow keys. Reference locations in the previous frames are shown as circle outlines. You should add as many reference locations as possible. However, you can safely skip some frames. Especially if the reference object stays at the same position. Now we added all reference locations. Let’s open the timelines again. White lines mark the reference locations. Play the video to see all reference locations. Turn off manual edit mode. To generate gaze from the reference locations, click “Calculate All Calibrations and Mappings”. Wait a little until the gaze is mapped. Now we see the gaze point. That’s it! You successfully used manual reference locations to map gaze!